| Autor: |
Gupteshwar Gupta, Vikram Sahai, Rajinder K. Gupta |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
BioResources, Vol 9, Iss 4, Pp 5801-5816 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1930-2126 |
| DOI: |
10.15376/biores.9.4.5801-5816 |
| Popis: |
An extracellular xylanase from the thermophilic fungus Melanocarpus albomyces IIS 68 was evaluated for its activity and stability in the presence of polyols and salts at 60 °C, and found to be an effective protecting agent for thermal deactivation of enzyme. Response surface methodology was employed to study the synergistic effects of glycerol and NaCl (thermo-stabilizers) for xylanase stability. The addition of these thermo-stabilizers resulted in more than a 10-fold increment in enzyme half-life. Activation energy (Ea) and thermodynamic parameters such as ∆H, ∆G, and ∆S were calculated for the thermal inactivation of free and immobilized xylanase. The immobilized enzyme underwent substantially less conformational changes because of its enhanced stability and increased compactness, providing better thermo-stability at elevated temperatures. These findings suggest that the combined effect of glycerol and sodium chloride serves as a potential stabilizer for extracellular thermophilic xylanase, which finds commercial application in many industries, especially in the pulp and paper industry. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
