Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation

Autor:	Bashu Dev Pardhe, Hackwon Do, Chang-Sook Jeong, Ki-Hwa Kim, Jun Hyuck Lee, Tae-Jin Oh
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	cyp105d18 papaverine n-oxide h2o2 tolerance streptomyces laurentii enzyme mechanisms crystal morphology co-crystals Crystallography QD901-999
Zdroj:	IUCrJ, Vol 8, Iss 4, Pp 684-694 (2021)
Druh dokumentu:	article
ISSN:	2052-2525 20522525 56105444
DOI:	10.1107/S2052252521005522
Popis:	The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The catalytic efficiency of CYP105D18 for papaverine N-oxidation was 1.43 s−1 µM−1. The heme oxidation rate (k) was low (
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/72335ce561054445804f68fb56a4442e Zobrazit plný text záznamu View record in DOAJ