GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS
| Autor: | Carmen Gentimir (Amititeloaie), Anda Cătălina Zaharia, Eugen Bitere, Dragoş Acatrinei, Ionuţ Cazan, Marcel Costuleanu, Otilia Boişteanu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Romanian Journal of Oral Rehabilitation, Vol 12, Iss 4, Pp 51-56 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2066-7000 2601-4661 |
| Popis: | Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when there is a close interaction of these cells. The experimental plan of the actual studies was represented by induced effects of the presence of gingival fibroblasts in co-culture on NAK-1 receptors-activated pro-lymphocytes B apoptosis. Results We clearly showed that murine pro-B lymphocyte apoptosis induced by indol-3-carbinol could be significantly enhanced by the presence of gingival fibroblasts in co-culture. Furthermore, the addition of 3-methyl-adenine in the medium for 24 hours blocked the enhancing effects induced by fibroblasts on the apoptotic index of pro-B lymphocytes. Conclusions So, the enhancing apoptotic effects of fibroblasts on pro-B lymphocytes are related to released autophagy-derived metabolites. Their real nature remains to be established through future researches involving this complex system. |
| Databáze: | Directory of Open Access Journals |
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