| Autor: |
Lin Wei, Yingying Xu, Min Du, Ying Fan, Ruyi Zou, Xiangyu Xu, Qingdong Zhang, Yu-Zhong Zhang, Wenshuang Wang, Fuchuan Li |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Data in Brief, Vol 48, Iss , Pp 109139- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3409 |
| DOI: |
10.1016/j.dib.2023.109139 |
| Popis: |
The data shown in this article are related to the published paper entitled “A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate” in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purification, specificity and biochemical characteristics of the identified chondroitin sulfate/dermatan sulfate 4-O-endosulfatase (endoBI4SF) are described in detail. The recombinant endoBI4SF with a molecular mass of 59.13 kDa can can specifically hydrolyze the 4-O- but not 2-O- and 6-O-sulfate groups in the oligo-/polysaccharides of chondroitin sulfate/dermatan sulfate and show the maximum reaction rate in 50 mM Tris-HCl buffer (pH 7.0) at 50°C, which can be a very useful tool for the structural and functional studies of chondroitin sulfate/dermatan sulfate. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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