| Autor: |
Wentao Zheng, Yandong Xia, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu, Ruijuan Li, Jia Yin |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Engineering Microbiology, Vol 2, Iss 4, Pp 100046- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-3703 |
| DOI: |
10.1016/j.engmic.2022.100046 |
| Popis: |
Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a compact Cascade-Cas3 system from P. aeruginosa (PaeCas3c) with a Pseudomonas-specific PEHR system, and the results of our recombineering assay showed that this compact Cascade-Cas3 system can significantly improve PEHR recombineering accuracy. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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