Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product

Autor: Lucia F. Zacchi, Dinora Roche-Recinos, Cassandra L. Pegg, Toan K. Phung, Mark Napoli, Campbell Aitken, Vanessa Sandford, Stephen M. Mahler, Yih Yean Lee, Benjamin L. Schulz, Christopher B. Howard
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-19 (2021)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-01903-x
Popis: Zacchi et al. attempt to bridge the gap between understanding the impact of process variables and determination of critical post-translational modifications on a highly complex glycoprotein, factor IX. They implement an LC-MS/MS approach based on data independent acquisition which allows measurement of all detectable peptides, even with heterogeneous modifications.
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