Indocyanine green-loaded exosomes for image-guided glioma nano-therapy

Autor: Bo Fan, Song Yang, Yuan-yu Wang, Chao Zhang, Ji-peng Yang, Li-qun Wang, Zhong-qiang Lv, Xue-fang Shi, Zhen-zeng Fan, Jian-kai Yang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Experimental Nanoscience, Vol 17, Iss 1, Pp 187-196 (2022)
Druh dokumentu: article
ISSN: 17458080
1745-8099
1745-8080
DOI: 10.1080/17458080.2022.2060500
Popis: Glioblastoma multiforme (GBM) is the most aggressive and deadliest type of brain tumor with a > >th he most aggressive and deadliest type of brain tumor witnear-infrared fluorescent dye, but it is hard to directly target brain tumor. Therefore, our study aimed to develop a new carrier for ICG to accurately visualize cancer tissue during surgery. Exosomes were isolated from culture U-87 MG cells, then loaded with Indocyanine green (ICG) and conjugated with RGERPPR peptide (RGE) (R-exo-ICG). The parameters of R-exo-ICG were analyzed and injected into tumor-bearing nude mice to evaluate its anti-tumor effect. R-exo-ICGs were nano size, and completely released ICG within 24 h. RGE modification increased cell uptake of exosomes, and induced cell apoptosis through activating Caspase 3 signaling pathway after laser irradiation. Moreover, R-exo-ICG was largely accumulated in the brain tumor in vivo, and inhibited tumor growth in tumor-bearing mice. RGE-conjugated exosomes that loaded ICG could target brain tumor in mice and significantly inhibited tumor growth in mice, which suggested that R-exo-ICG might serve as a novel approach for GBM treatment.
Databáze: Directory of Open Access Journals
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