2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.
| Autor: | Ivanilda dos Santos Alves, Valéria Cristina Barbosa Carmazini, Cosme Damião dos Santos, Julieta Andrea Silva de Almeida |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 48, Iss 11 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/0103-8478cr20180001 |
| Popis: | ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively. |
| Databáze: | Directory of Open Access Journals |
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