Encapsulation of human serum albumin in submicrometer magnetic poly(lactide-co-glycolide) particles as a model system for targeted drug delivery

Autor: Shubhra Quazi T.H., Macková Hana, Horák Daniel, Fodor-Kardos Andrea, Tóth Judit, Gyenis János, Feczkó Tivadar
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: e-Polymers, Vol 2013, Iss 1, Pp 310-318 (2013)
Druh dokumentu: article
ISSN: 1618-7229
DOI: 10.1515/epoly.2013.2013.1.310
Popis: Two types of iron oxide nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) chlorides: water-dispersible γ-Fe2O3 and organic solvent-dispersible oleic acid-coated Fe3O4 particles. The nanoparticles, together with human serum albumin (HSA) serving as a model for a protein-type drug, were then incorporated in poly(lactide-co-glycolide) (PLGA) particles using double emulsion solvent evaporation technique. Morphology, size and particle size distribution of the resulting particles was analyzed by electron microscopy and dynamic light scattering. Iron oxide and HSA encapsulating efficiency was determined by Prussian Blue staining and micro-BCA assay, respectively.
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