Diffusion-tensor magnetic resonance imaging as a non-invasive assessment of extracellular matrix remodeling in lumbar paravertebral muscles of rats with sarcopenia

Autor: Xin-Chen Huang, Ji-Yao Ma, Chao Gao, Jia-Xin Chen, Chun-Li Li, Yi-Long Huang, Bo He
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: BMC Musculoskeletal Disorders, Vol 25, Iss 1, Pp 1-11 (2024)
Druh dokumentu: article
ISSN: 1471-2474
02739917
DOI: 10.1186/s12891-024-07654-0
Popis: Abstract Background Extracellular matrix (ECM) remodeling in skeletal muscle is a significant factor in the development of sarcopenia. This study aims to evaluate changes in ECM remodeling in the lumbar paravertebral muscles of sarcopenic rats using diffusion-tensor magnetic resonance imaging (DT-MRI) and compare them with histology. Methods Twenty 6-month-old female Sprague Dawley rats were randomly divided into the dexamethasone (DEX) group and the control (CON) group. Both groups underwent 3.0T MRI scanning, including Mensa, T2WI, and DT-MRI sequences. The changes in muscle fibers and extracellular matrix (ECM) of the erector spinal muscle were observed using hematoxylineosin and sirius red staining. The expressions of collagen I, III, and fibronectin in the erector spinae were detected by western blot. Pearson correlation analysis was employed to assess the correlation between MRI quantitative parameters and corresponding histopathology markers. Results The cross-sectional area and fractional anisotropy values of the erector spinae in the DEX group rats were significantly lower than those in the CON group (p
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