| Autor: |
Giovanna Petrucci, Alessandro Rizzi, Simone Bellavia, Francesco Dentali, Giovanni Frisullo, Dario Pitocco, Paola Ranalli, Pier Andrea Rizzo, Irene Scala, Mauro Silingardi, Elisa Zagarrì, Gualberto Gussoni, Bianca Rocca |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Research and Practice in Thrombosis and Haemostasis, Vol 8, Iss 8, Pp 102623- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2475-0379 |
| DOI: |
10.1016/j.rpth.2024.102623 |
| Popis: |
Background: Serum thromboxane B2 (sTXB2) is a validated biomarker of low-dose aspirin pharmacodynamics. In the original method, nonanticoagulated blood samples must be incubated at 37 °C immediately after withdrawal, centrifuged and serum supernatant should be frozen until assayed. Timely completion of all preanalytical steps may affect the feasibility and quality of sTXB2 measurements. The storage duration of frozen serum can also affect sTXB2 stability. Objectives: We assessed the stability of sTXB2 in clotted blood samples stored at 4 °C before further processing and in sera stored at −40 °C for over a decade. Methods: Venous whole blood withdrawn from individuals on chronic low-dose aspirin was dispensed in different tubes and immediately incubated at 37 °C for 1 hour. The reference tube was promptly processed following the original protocol; the remaining tubes were stored at 4 °C for 12 to 72 hours before further processing. Sera stored at a controlled −40 °C temperature for |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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