Alternative animal model for studies of total skin thickness burns

Autor: Ana Laura Martins de Andrade, Julia Risso Parisi, Patrícia Brassolatti, Nivaldo Antonio Parizotto
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Acta Cirúrgica Brasileira, Vol 32, Iss 10, Pp 836-842 (2017)
Druh dokumentu: article
ISSN: 0102-8650
DOI: 10.1590/s0102-865020170100000005
Popis: Abstract Purpose: To present an alternative experimental model of third degree burn of easy reproducibility. Methods: Eighteen male Wister rats were randomly divided into three groups, 6 of which were allocated to each group. A soldering iron coupled to an aluminum plate was used to produce burn, at a temperature of 150ºC, with different exposure times per group. Group 5 (G5) animals were burned at 150°C with exposure time of 5 seconds; Group 10 (G10) the animals were burned at 150°C with exposure time of 10 seconds and group 15 (G15) the animals were burned at 150°C with exposure time of 15 seconds. Results: Histopathological analyzes showed that all three groups had similar morphological characteristics, with total thickness involvement. Conclusion: The technique is effective to reproduce a third degree burn and suggests the temperature of 150ºC with 5 seconds of exposure in order to minimize the risks to the animals.
Databáze: Directory of Open Access Journals