Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

Autor: Ricardo D. Righetto, Nikhil Biyani, Julia Kowal, Mohamed Chami, Henning Stahlberg
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-019-09661-5
Popis: 2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to determine the structure at 4 Å resolution.
Databáze: Directory of Open Access Journals