Embryonic stem cells overexpressing high molecular weight FGF2 isoform enhance recovery of pre-ganglionic spinal root lesion in combination with fibrin biopolymer mediated root repair

Autor:	B. H. M. Lima, L. P. Cartarozzi, S. Kyrylenko, R. S. Ferreira, B. Barraviera, Alexandre L. R. Oliveira
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	Cell therapy Embryonic stem cells Fibroblast growth factor 2 Motoneurons Spinal cord injury Medicine (General) R5-920 Biochemistry QD415-436
Zdroj:	Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-20 (2024)
Druh dokumentu:	article
ISSN:	1757-6512
DOI:	10.1186/s13287-024-03676-6
Popis:	Abstract Background Spinal ventral root avulsion results in massive motoneuron degeneration with poor prognosis and high costs. In this study, we compared different isoforms of basic fibroblast growth factor 2 (FGF2), overexpressed in stably transfected Human embryonic stem cells (hESCs), following motor root avulsion and repair with a heterologous fibrin biopolymer (HFB). Methods In the present work, hESCs bioengineered to overexpress 18, 23, and 31 kD isoforms of FGF2, were used in combination with reimplantation of the avulsed roots using HFB. Statistical analysis was conducted using GraphPad Prism software with one-way or two-way ANOVA, followed by Tukey’s or Dunnett’s multiple comparison tests. Significance was set at *p
Databáze:	Directory of Open Access Journals
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