| Autor: |
G. Lucchetta, M. Sorgato, E. Zanchetta, G. Brusatin, E. Guidi, R. Di Liddo, M. T. Conconi |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
eXPRESS Polymer Letters, Vol 9, Iss 4, Pp 354-361 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1788-618X |
| DOI: |
10.3144/expresspolymlett.2015.33 |
| Popis: |
In this work, osteoinductive micro-pillared polystyrene surfaces were mass-produced for bone replacement applications, by means of the micro injection molding process. Firstly, the molding process parameters were optimized with a two-level, three-factor central composite face-centered plan to increase the quality of polystyrene micro pillars replication and to maximize the pillars height uniformity over the molded part. Secondly, osteoblastic MC3T3-E1 cells adhesion and proliferation on the replicated substrates were assessed as a function of micro topography parameters, such as pillars diameter, aspect ratio and spacing. Cell morphology and proliferation were evaluated through MTS test after 1, 3 and 7 days from seeding. The experimental results showed that cells adhesion and proliferation is more positively promoted on micro-pillared surfaces compared to flat surfaces, but no correlations were observed between cell proliferation and pillar diameter and spacing. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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