HPLC-DAD Development and Validation Method for Short-Chain Fatty Acids Quantification from Chicken Feces by Solid-Phase Extraction

Autor: Lenin Rodolfo Díaz-Corona, Karina Jeanette Parra-Saavedra, Renata Sofia Mora-Alonzo, María Esther Macías-Rodríguez, Alma H. Martínez-Preciado, Santiago José Guevara-Martínez, Adalberto Zamudio-Ojeda, Adriana Macaria Macias-Lamas
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Separations, Vol 10, Iss 5, p 308 (2023)
Druh dokumentu: article
ISSN: 2297-8739
DOI: 10.3390/separations10050308
Popis: A solid-phase extraction (SPE) process, followed by an HPLC-DAD method, was developed and validated to quantify short-chain fatty acids (SCFAs) and applied to analyze chicken feces samples. This study aimed to report the use of the STRATATM-X-A 96 Well Plate SPE cartridge method as the first step in quantifying SCFAs. A stationary reverse-phase Luna Omega C18 column kept at 40 °C was used, with a gradient elution mobile phase (H2SO4 and Acetonitrile, 98:2), a flow rate of 1.2 mL/min, and detection at 210 nm. A mixture of acetic, propionic, and butyric acid was used as thestandard. The method showed a linear relationship, with a coefficient of determination of R2 = 0.9987, R2 = 0.9985, and R2 = 0.9966 for acetic, propionic, and butyric acid, respectively. Concerning sensitivity, an LOD and LOQ of 0.14, 0.14, 0.14 mg/mL and 0.44, 0.45, 0.43 mg/mL were obtained for acetic, propionic, and butyric acid, respectively. According to the sample analysis, the accuracy was 76.05, 95.60, and 81.56% for acetic, propionic, and butyric acid, respectively. The developed method is simple, fast, linear, sensitive, accurate, precise, and robust for the quantification of SCFAs. This could serve as an alternative to conventional methodologies for the determination of these critical components in the intestinal health of chicken feces.
Databáze: Directory of Open Access Journals
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