CATECHOLAMINE ASSAY BY RADIOENZYMATIC METHOD - A PROTOCOL
| Autor: | Nasim Karim, Joshua R. Berlin |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Journal of the Dow University of Health Sciences, Vol 2, Iss 3, Pp 121-126 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1995-2198 2410-2180 |
| Popis: | Catecholamines are a group o f compounds identified by the catechol nucleus. The significant catecholaminesfound naturally in the body are epinephrine, nor- epinephrine and dopamine. They are found in varioustissues o f the body like brain, adrenals, GIT, blood, urine and cerebrospinal fluid in picogram quantities.Knowledge of catecholamines is important in the diagnosis and management o f pheochromocytoma, CNStumours like neuroblastoma, Diabetes mellitus, hypertension, coronary disease, angina pectoris, myocardialinfarction etc. Analytical techniques for determination o f catecholamines have been developed which aregenerally based upon the use o f isolated enzyme catechol-O-methyltransferase (COMT) to transfer aradioactive methyl group from S-adenosyl-L-methionine (SAM) to an endogenous catecholamine acceptormolecule to form a radioactive O-methyl catecholamine derivative. This article gives a basic out line ofthe catecholamine assay along with a simplified protocol that can be easily performed in the laboratory. |
| Databáze: | Directory of Open Access Journals |
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