| Autor: |
Chao Wu, Abraham J. Qavi, Asmaa Hachim, Niloufar Kavian, Aidan R. Cole, Austin B. Moyle, Nicole D. Wagner, Joyce Sweeney-Gibbons, Henry W. Rohrs, Michael L. Gross, J. S. Malik Peiris, Christopher F. Basler, Christopher W. Farnsworth, Sophie A. Valkenburg, Gaya K. Amarasinghe, Daisy W. Leung |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
iScience, Vol 24, Iss 6, Pp 102681- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2589-0042 |
| DOI: |
10.1016/j.isci.2021.102681 |
| Popis: |
Summary: Nucleocapsid (N) encoded by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays key roles in the replication cycle and is a critical serological marker. Here, we characterize essential biochemical properties of N and describe the utility of these insights in serological studies. We define N domains important for oligomerization and RNA binding and show that N oligomerization provides a high-affinity RNA-binding platform. We also map the RNA-binding interface, showing protection in the N-terminal domain and linker region. In addition, phosphorylation causes reduction of RNA binding and redistribution of N from liquid droplets to loose coils, showing how N-RNA accessibility and assembly may be regulated by phosphorylation. Finally, we find that the C-terminal domain of N is the most immunogenic, based on antibody binding to patient samples. Together, we provide a biochemical description of SARS-CoV-2 N and highlight the value of using N domains as highly specific and sensitive diagnostic markers. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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