Position-dependent correlation between TBX22 exon 5 methylation and palatal shelf fusion in the development of cleft palate
| Autor: | KE LI, XUAN SHU, HUI GONG, LIUHANGHANG CHENG, ZEJUN DONG, SHENYOU SHU |
|---|---|
| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Anais da Academia Brasileira de Ciências, Vol 91, Iss 2 |
| Druh dokumentu: | article |
| ISSN: | 0001-3765 1678-2690 |
| DOI: | 10.1590/0001-3765201920180945 |
| Popis: | Abstract: DNA methylation is essential for spatiotemporally-regulated gene expression in embryonic development. TBX22 (Chr X: 107667964-107688978) functioning as a transcriptional repressor affects DNA binding, sumoylation, and transcriptional repression associated with X-linked cleft palate. This study aimed to explore the relationship and potential mechanism between TBX22 exon 5 methylation and palatal shelf fusion induced by all-trans retinoic acid (ATRA). We performed DNA methylation profiling, using MethylRAD-seq, after high throughput sequencing of mouse embryos from control (n=9) and ATRA-treated (to induce cleft palate, n=9) C57BL/6J mice at embryonic gestation days(E) 13.5, 14.5 and 16.5. TBX22 exon 5 was hyper-methylated at the CpG site at E13.5 (P=0.025, log2FC=1.5) and E14.5 (P=0.011, log2FC:1.5) in ATRA-treated, whereas methylation TBX22 exon 5 at the CpG site was not significantly different at E16.5 (P=0.808, log2FC=-0.2) between control and ATRA-treated. MSP results showed a similar trend consistent with the MethylRAD-seq results. qPCR showed the change in TBX22 exon 5 expression level negatively correlated with its TBX22 exon 5 methylation level. These results indicate that changes in TBX22 exon 5 methylation might play an important regulatory role during palatal shelf fusion, and may enlighten the development of novel epigenetic biomarkers in the treatment of CP in the future. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|