| Autor: |
Ming PANG, Xiong GUAN, Chen-Xiang ZUO, Ming-Jie LIU, Saad REHMAN, Qin-Lei FAN, Ping LU, De-Kun CHEN, Wen-Tao MA |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Kafkas Universitesi Veteriner Fakültesi Dergisi, Vol 26, Iss 1, Pp 71-74 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1309-2251 |
| DOI: |
10.9775/kvfd.2019.22287 |
| Popis: |
This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigens for mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further results showed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunofluorescence analysis using this mAb showed increased milk αs1-casein level in mastitis-affected goats. In conclusion, this study has established an effective tool to evaluate the expression level of αs1-casein during mastitis development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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