Characterization of newly isolated thermotolerant bacterium Cupriavidus sp. CB15 from composting and its ability to produce polyhydroxyalkanoate from glycerol

Autor: Anuyut Yootoum, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Churairat Moukamnerd, Thanongsak Chaiyaso, Chayakorn Pumas, Nuttapol Tanadchangsaeng, Masanori Watanabe, Toshiaki Fukui, Chayatip Insomphun
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Microbial Cell Factories, Vol 22, Iss 1, Pp 1-15 (2023)
Druh dokumentu: article
ISSN: 1475-2859
DOI: 10.1186/s12934-023-02059-5
Popis: Abstract Background This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions. Results A newly thermotolerant polyhydroxyalkanoate (PHA) producing bacterium, Cupriavidus sp. strain CB15, was isolated from corncob compost. The potential ability to synthesize PHA was confirmed by detection of PHA synthase (phaC) gene in the genome. This strain could produce poly(3-hydroxybutyrate) [P(3HB)] with 0.95 g/L (PHA content 75.3 wt% of dry cell weight 1.24 g/L) using glycerol as a carbon source. The concentration of PHA was enhanced and optimized based on one-factor-at-a-time (OFAT) experiments and response surface methodology (RSM). The optimum conditions for growth and PHA biosynthesis were 10 g/L glycerol, 0.78 g/L NH4Cl, shaking speed at 175 rpm, temperature at 45 °C, and cultivation time at 72 h. Under the optimized conditions, PHA production was enhanced to 2.09 g/L (PHA content of 74.4 wt% and dry cell weight of 2.81 g/L), which is 2.12-fold compared with non-optimized conditions. Nuclear magnetic resonance (NMR) analysis confirmed that the extracted PHA was a homopolyester of 3-hydyoxybutyrate. Conclusion Cupriavidus sp. strain CB15 exhibited potential for cost-effective production of PHA from glycerol.
Databáze: Directory of Open Access Journals
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