| Autor: |
Naveen Kumar, Ashutosh Singh, Preeti Dhaka, Ankur Singh, Pragya Agarwala, Kuldeep Sharma, Anudita Bhargava, Sandeep Bhatia, Thomas Launey, Rahul Kaushik, Shailly Tomar, Aniket Sanyal |
| Jazyk: |
angličtina |
| Rok vydání: |
2025 |
| Předmět: |
|
| Zdroj: |
Sensing and Bio-Sensing Research, Vol 47, Iss , Pp 100718- (2025) |
| Druh dokumentu: |
article |
| ISSN: |
2214-1804 |
| DOI: |
10.1016/j.sbsr.2024.100718 |
| Popis: |
Given the global implementation of effective COVID-19 vaccines, which do not confer complete immunity, it is crucial to monitor the occurrence of breakthrough infections, particularly against newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Hence, we developed a label-free colorimetric assay using gold nanoparticles (GNPs) functionalized with a peptide dendrimer incorporating highly reactive epitopes of the nucleocapsid (N) protein. This assay relies on the tween-20 induced colorimetric changes caused by the aggregation of peptide dendrimer-coated GNPs in the absence of anti- SARS-CoV-2 N antibodies, and vice versa. Transmission electron microscopy, dynamic light scattering, and circular dichroism spectroscopy analyses all showed the formation of a uniform and highly stable coating of the peptide dendrimer over GNPs. Surface plasmon resonance experiments have demonstrated a strong binding affinity for the peptide dendrimer and anti- SARS-CoV-2 N antibodies, with a KD value of 525 nM. To validate the proof-of-concept, we have tested this assay on seventy human serum samples, and receiver operating characteristic curve analysis demonstrated high diagnostic sensitivity (88.89 %) and specificity (100 %). This approach opens up new avenues for the development of simple and rapid diagnostic assays for identifying antibodies against viral infections and other pathogens. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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