SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway
| Autor: | Wenfei Gu, GuangTao Qi, Li Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Iranian Journal of Immunology, Vol 20, Iss 3, Pp 335-347 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1735-1383 1735-367X |
| DOI: | 10.22034/iji.2023.98236.2559 |
| Popis: | Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity.Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells.Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays.Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p |
| Databáze: | Directory of Open Access Journals |
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