Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment

Autor: Laura Sanchis-Artero, Juan Francisco Martínez-Blanch, Sergio Manresa-Vera, Ernesto Cortés-Castell, Marina Valls-Gandia, Marisa Iborra, Jose Maria Paredes-Arquiola, Maia Boscá-Watts, Jose Maria Huguet, Rafael Gil-Borrás, Josefa Rodríguez-Morales, Xavier Cortés-Rizo
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-021-88823-2
Popis: Abstract Intestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational study involved 27 CD patients initiating anti-TNF treatment and 16 healthy individuals. Inflammatory activity was determined at baseline, 3 and 6 months, classifying patients into responders and non-responders. Fecal microbiota was analyzed by massive genomic sequencing thought 16S rRNA amplicon sequencing before and after six months of anti-TNF treatment. The CD cohort showed a decrease in genera of the class Clostridia, short-chain fatty acid producers, and an increase in the phylum Proteobacteria (p
Databáze: Directory of Open Access Journals
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