A new human adipocyte model with PTEN haploinsufficiency
| Autor: | Franziska Kässner, Anna Kirstein, Norman Händel, Gordian L. Schmid, Kathrin Landgraf, Antje Berthold, Astrid Tannert, Michael Schaefer, Martin Wabitsch, Wieland Kiess, Antje Körner, Antje Garten |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Adipocyte, Vol 9, Iss 1, Pp 290-301 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2162-3945 2162-397X 21623945 |
| DOI: | 10.1080/21623945.2020.1785083 |
| Popis: | Few human cell strains are suitable and readily available as in vitro adipocyte models. We used resected lipoma tissue from a patient with germline phosphatase and tensin homolog (PTEN) haploinsufficiency to establish a preadipocyte cell strain termed LipPD1 and aimed to characterize cellular functions and signalling pathway alterations in comparison to the established adipocyte model Simpson-Golabi-Behmel-Syndrome (SGBS) and to primary stromal-vascular fraction cells. We found that both cellular life span and the capacity for adipocyte differentiation as well as adipocyte-specific functions were preserved in LipPD1 and comparable to SGBS adipocytes. Basal and growth factor-stimulated activation of the PI3 K/AKT signalling pathway was increased in LipPD1 preadipocytes, corresponding to reduced PTEN levels in comparison to SGBS cells. Altogether, LipPD1 cells are a novel primary cell model with a defined genetic lesion suitable for the study of adipocyte biology. |
| Databáze: | Directory of Open Access Journals |
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