| Autor: |
Alexandre Ho-Pun-Cheung, Hervé Bazin, Nadège Gaborit, Christel Larbouret, Patrick Garnero, Eric Assenat, Florence Castan, Caroline Bascoul-Mollevi, Jeanne Ramos, Marc Ychou, André Pèlegrin, Gérard Mathis, Evelyne Lopez-Crapez |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
PLoS ONE, Vol 7, Iss 7, p e37065 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0037065 |
| Popis: |
Following the development of targeted therapies against EGFR and HER2, two members of the human epidermal receptor (HER) family of receptor tyrosine kinases, much interest has been focused on their expression in tumors. However, knowing the expression levels of individual receptors may not be sufficient to predict drug response. Here, we describe the development of antibody-based time-resolved Förster resonance energy transfer (TR-FRET) assays for the comprehensive analysis not only of EGFR and HER2 expression in tumor cryosections, but also of their activation through quantification of HER homo- or heterodimers. First, EGFR and HER2 expression levels were quantified in 18 breast tumors and the results were compared with those obtained by using reference methods. The EGFR number per cell determined by TR-FRET was significantly correlated with EGFR mRNA copy number (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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