Effect of Perfluorane on the Amplitude-Frequency Spectrum of Fluctuations in Cerebral Blood Flow in Hemorrhagic Hypotension and During the Reperfusion Period
| Autor: | I. A. Ryzhkov, Yu. V. Zarzhetsky, I. S. Novoderzhkina |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Общая реаниматология, Vol 11, Iss 4, Pp 14-22 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1813-9779 2411-7110 |
| DOI: | 10.15360/1813-9779-2015-4-14-22 |
| Popis: | Objective: to use laser Doppler flowmetry (LDF) to investigate the effect of perfluorane on the time course of microhemocirculato ry changes in the rat pial vessels in acute blood loss and after autohemotransfusion.Material and methods. Experiments were car ried out on 31 outbred male rats weighing 300—400 g under anesthesia with intraperitoneal Nembutal 45 mg/kg. The caudal artery was catheterized to measure blood pressure (BP), to sample and reinfuse blood, and to administer infusion solutions. LDF (ЛАКК 02 device, LAZMA, Russia) was used to record blood flow in the pial vessels of the left parietal region (the center coordinates were 3 mm caudal to bregma and 2 mm left of the sagittal suture. A volumefixed acute blood loss model was applied. The goal amount of blood loss was 30% of the circulating blood volume. At 10 minutes after blood sampling, the animals were administered 0.9% NaCl solution (physiologic saline (PS), n=15) or perfluorane (PF), n=16)) in a dose of 3 ml/kg body weight. At 60 minutes following blood sampling, autohemotransfusion was used, after which there was a 60min reperfusion period. The investigators analyzed LDF readings and determined the following indicators: microcirculation index; the maximum amplitudes of blood flow fluctuations in the endothelial, neurogenic, and myogenic frequency ranges by a wavelet analysis. The data were statistically processed using Statistica 7.0 software.Results. Hypovolemia caused a more than 50% reduction in BP; moreover, blood flow in the pial vessels decreased by less than 20% of its baseline level (p |
| Databáze: | Directory of Open Access Journals |
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