Prevalence of Extended-Spectrum β-Lactamases in E. coli of Rats in the Region North East of Gabon

Autor: Richard Onanga, Pierre Philippe Mbehang Nguema, Guy Roger Ndong Atome, Arsène Mabika Mabika, Berthelemy Ngoubangoye, Wed Leslie Komba Tonda, Jean Constant Obague Mbeang, Jacques Lebibi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Veterinary Medicine International, Vol 2020 (2020)
Druh dokumentu: article
ISSN: 2090-8113
2042-0048
DOI: 10.1155/2020/5163493
Popis: Antibiotic resistance occurs in the environment by multiplication and the spread of multidrug-resistant bacteria that would be due to an improper and incorrect use of antibiotics in human and veterinary medicine. The aim of this study was to establish the prevalence of E.coli producing Extended-Spectrum beta-Lactamase (ESBL) antibiotics from rats and gregarious animals in a semirural area of Gabon and to evaluate the origin of a resistance distribution in the environment from animal feces. The bacterial culture was carried out, and the identification of E. coli strains on a specific medium and the antibiotic susceptibility tests allowed establishing the prevalence. Characterization of resistance genes was performed by gene amplification after DNA extraction. On 161 feces collected in rats, 32 strains were isolated, and 11 strains of E. coli produced ESBL with a prevalence of 34.37%. Molecular tests showed that CTX-M genes 214 bp were identified in rats. The presence of CTX-M genes could have a human origin. So, the rats can carry ESBL-producing Enterobacteriaceae which poses a risk to human health and pets in this region of Gabon.
Databáze: Directory of Open Access Journals
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