Diversity Analysis and Genetic Relationships among Local Brazilian Goat Breeds Using SSR Markers

Autor: Marcos Paulo Carrera Menezes, Amparo Martinez Martinez, Edgard Cavalcanti Pimenta Filho, Jose Luis Vega-Pla, Juan Vicente Delgado, Janaina Kelli Gomes Arandas, Laura Leandro da Rocha, Maria Norma Ribeiro
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Animals, Vol 10, Iss 10, p 1842 (2020)
Druh dokumentu: article
ISSN: 2076-2615
DOI: 10.3390/ani10101842
Popis: The genetic diversity of six Brazilian native goats was reported using molecular markers. Hair samples of 332 animals were collected from different goat breeds (Moxotó, Canindé, Serrana Azul, Marota, Repartida, and Graúna) from five states of Northeast Brazil (Paraíba, Pernambuco, Rio Grande do Norte, Bahia, and Piauí). A panel of 27 microsatellites or single sequence repeats (SSRs) were selected and amplified using a polymerase chain reaction (PCR) technique. All populations showed an average allele number of over six. The mean observed heterozygosity for Brazilian breeds was superior to 0.50. These results demonstrated the high genetic diversity in the studied populations with values ranging from 0.53 (Serrana Azul) to 0.62 (Repartida). The expected average heterozygosity followed the same trend ranging from 0.58 (Serrana Azul) to 0.65 (Repartida), and the values obtained are very similar for all six breeds. The fixation index (Fis) had values under 10% except for the Moxotó breed (13%). The mean expected heterozygosity of all Brazilian populations was over 0.50. Results indicated a within-breed genetic variability in the Brazilian breeds based on the average number of alleles and the average observed heterozygosity. The interbreed genetic diversity values showed proper genetic differentiation among local Brazilian goat breeds.
Databáze: Directory of Open Access Journals
Nepřihlášeným uživatelům se plný text nezobrazuje