Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1

Autor:	Jasenko Zivanov, Takanori Nakane, Sjors H. W. Scheres
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	cryo-em relion aberrations anisotropic magnification Crystallography QD901-999
Zdroj:	IUCrJ, Vol 7, Iss 2, Pp 253-267 (2020)
Druh dokumentu:	article
ISSN:	2052-2525 20522525
DOI:	10.1107/S2052252520000081
Popis:	Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be used to correct for these aberrations for any given cryo-EM data set, a posteriori. Using five publicly available data sets, it is shown that considering these aberrations improves the resolution of the 3D reconstruction when these effects are present. The methods are implemented in version 3.1 of the open-source software package RELION.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/70365abd15de459989db61c9573348cf Zobrazit plný text záznamu View record in DOAJ