Autor: |
Feifei Ma, Ranzhe Li, Guanghui Guo, Fang Nie, Lele Zhu, Wenjuan Liu, Linlin Lyu, Shenglong Bai, Xinpeng Zhao, Zheng Li, Dale Zhang, Hao Li, Suoping Li, Yun Zhou, Chun-Peng Song |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
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Zdroj: |
Crop Journal, Vol 11, Iss 5, Pp 1521-1532 (2023) |
Druh dokumentu: |
article |
ISSN: |
2214-5141 |
DOI: |
10.1016/j.cj.2023.05.001 |
Popis: |
To break the narrow diversity bottleneck of the wheat D genome, a set of Aegilops tauschii-wheat introgression (A-WI) lines was developed by crossing Ae. tauschii accession T015 with common wheat elite cultivar Zhoumai 18 (Zhou18). A high-density genetic map was constructed based on Single Nucleotide Polymorphism (SNP) markers and 15 yield-related traits were evaluated in 11 environments for detecting quantitative trait loci (QTL). A total of 27 environmentally stable QTL were identified in at least five environments, 20 of which were derived from Ae. tauschii T015, explaining up to 24.27% of the phenotypic variations. The major QTL for kernel length (KL), QKl-2D.5, was delimited to a physical interval of approximately 2.6 Mb harboring 52 candidate genes. Three Kompetitive Allele Specific PCR (KASP) markers were successfully developed based on nonsynonymous nucleotide mutations of candidate gene AetT093_2Dv1G100900.1 and showed that A-WI lines with the T015 haplotype had significantly longer KL than the Zhou18 haplotype across all 11 environments. Four primary valuable A-WIs with good trait performance and carrying yield-related QTL were selected for breeding improvement. The results will facilitate the efficient transfer of beneficial genes from Ae. tauschii into wheat cultivars to improve wheat yield and other traits. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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