Exploring Viral Metagenomics in Pediatric Patients with Acute Respiratory Infections: Unveiling Pathogens beyond SARS-CoV-2
| Autor: | Gabriel Montenegro de Campos, Debora Glenda Lima de La-Roque, Alex Ranieri Jerônimo Lima, Victória Simionatto Zucherato, Eneas de Carvalho, Loyze Paola Oliveira de Lima, Pedro de Queiroz Cattony Neto, Murilo Marconi dos Santos, Massimo Ciccozzi, Marta Giovanetti, Rodrigo Haddad, Luiz Carlos Júnior Alcantara, Maria Carolina Elias, Sandra Coccuzzo Sampaio, Dimas Tadeu Covas, Simone Kashima, Svetoslav Nanev Slavov |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Microorganisms, Vol 11, Iss 11, p 2744 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2076-2607 10429085 |
| DOI: | 10.3390/microorganisms11112744 |
| Popis: | The emergence of SARS-CoV-2 and the subsequent pandemic have prompted extensive diagnostic and clinical efforts to mitigate viral spread. However, these strategies have largely overlooked the presence of other respiratory viruses. Acute respiratory diseases in pediatric patients can be caused by a diverse range of viral agents, and metagenomics represents a powerful tool for their characterization. This study aimed to investigate the viral abundance in pediatric patients with acute respiratory symptoms who tested negative for SARS-CoV-2 during the Omicron pandemic wave. To achieve this, viral metagenomics and next-generation sequencing were employed on 96 nasopharyngeal swab samples, which were organized into 12 pools, with each pool consisting of eight individual samples. Metagenomic analysis revealed that the most prevalent viruses associated with acute disease in pediatric patients were respiratory syncytial virus (detected in all pools) and enteroviruses, which are known to cause significant morbidity and mortality in children. Additionally, clinically significant viruses such as mumps orthorubulavirus, human metapneumovirus, influenza A, and a wide array of human herpesviruses (1, 3–7) were identified. These findings highlight the extensive potential of viral metagenomics in identifying viruses other than SARS-CoV-2 that contribute to acute infections in children. Consequently, this methodology should garner clinical attention in terms of differential diagnosis and the development of public policies to address such conditions in the global pediatric population. |
| Databáze: | Directory of Open Access Journals |
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