| Autor: |
Kanchanamala Kanala, Nagiat T. Hwisa, Babu Rao Chandu, Prakash Katakam, Mukkanti Khagga, B.R. Challa, Bhavyasri Khagga |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Journal of Pharmaceutical Analysis, Vol 3, Iss 6, Pp 481-488 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
2095-1779 |
| DOI: |
10.1016/j.jpha.2013.03.009 |
| Popis: |
A simple, sensitive and specific liquid chromatographyâtandem mass spectrometry (LCâMS/MS) method was developed for the quantification of milnacipran (MC) in rat plasma by using the liquidâliquid extraction method. Milnacipran-d10 (MCD10) was used as an internal standard (IS). Chromatographic separation was achieved on Zorbax SB-CN (4.6 mmÃ75 mm, 3.5 µm) column with an isocratic mobile phase composed of 10 mM ammonium acetate (pH 4.0) and methanol in the ratio of 25:75(v/v), at a flow-rate of 0.7 mL/min. MC and MCD10 were detected with proton adducts at m/z 247.2â230.3 and m/z 257.2â240.4 in multiple reaction monitoring (MRM) positive mode respectively. The method was validated over a linear concentration range of 1.00â400.00 ng/mL with a correlation coefficient (r2)â¥0.9850. This method demonstrated intra- and inter-day precision within 5.40â10.85% and 4.40â8.29% and accuracy within 97.00â104.20% and 101.64â106.23%. MC was found to be stable throughout three freezeâthaw cycles, bench top and postoperative stability studies. This method was successfully applied to a pharmacokinetic study of rats through i.v. administration. Keywords: Milnacipran, Pharmacokinetics, Rat plasma, LCâMS/MS |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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