Multi-Focus Images Fusion for Fluorescence Imaging Based on Local Maximum Luminosity and Intensity Variance

Autor: Hao Cheng, Kaijie Wu, Chaochen Gu, Dingrui Ma
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Sensors, Vol 24, Iss 15, p 4909 (2024)
Druh dokumentu: article
ISSN: 1424-8220
DOI: 10.3390/s24154909
Popis: Due to the limitations on the depth of field of high-resolution fluorescence microscope, it is difficult to obtain an image with all objects in focus. The existing image fusion methods suffer from blocking effects or out-of-focus fluorescence. The proposed multi-focus image fusion method based on local maximum luminosity, intensity variance and the information filling method can reconstruct the all-in-focus image. Moreover, the depth of tissue’s surface can be estimated to reconstruct the 3D surface model.
Databáze: Directory of Open Access Journals
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