Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation

Autor: Oi Wah Liew, Samantha S. M. Ling, Shera Lilyanna, Yue Zhou, Peipei Wang, Jenny P. C. Chong, Yan Xia Ng, Angeline E. S. Lim, Eliot R. Y. Leong, Qifeng Lin, Teck Kwang Lim, Qinsong Lin, Enoch M. W. Ng, Tuck Wah Ng, A. Mark Richards
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-17 (2021)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-01965-x
Popis: Liew et al. describe an efficient monoclonal antibody (mAb) production method and as a proof-of-concept, develop mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.
Databáze: Directory of Open Access Journals
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