Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo Use of an enzymatic method for obtaining protein extracts from wheat flour
| Autor: | Raquel Linhares Carreira, Camila Salles Ramos, Mauro Ramalho Silva, Carlos de Oliveira Lopes Junior, Marialice Pinto Coelho Silvestre, Mônica Ribeiro Pirozi |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 32, Iss 4, Pp 919-922 (2009) |
| Druh dokumentu: | article |
| ISSN: | 0100-4042 1678-7064 |
| DOI: | 10.1590/S0100-40422009000400017 |
| Popis: | An enzymatic method was used for obtaining protein extracts from wheat flour using an alkaline protease. Some parameters were evaluated aiming the optimization of this method: temperature (40-50 ºC); time (2-5 h); physical treatment of the sample (no treatment, ultra-turrax/16,000 rpm/5 min and ultrasound/120 W/10 min); enzyme:substrate ratio (E:S) of 5:100 - 10:100 and concentration of wheat flour (1:3, 1:5 and 1:10 w/v). The results showed that the best condition for protein extraction was that using the sample concentration of 1:3 (w/v), ultra-turrax, E:S of 10:100, at 40 ºC, 2 h, having reached an extraction yield of 88.53%. |
| Databáze: | Directory of Open Access Journals |
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