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This study was conducted to evaluate the probiotic potentiality of GM-Lac (a mixture that comprises four lactic acid bacteria (LAB) strains– Limosilactobacillus fermentum GMNL-93, Lactiplantibacillus plantarum GMNL-141, Lacticaseibacillus casei GMNL-277, and Bifidobacterium lactis GMNL-550) applied in fish aquaculture. First, the four LAB isolates (GMNL-93, −141, −277, and −550) constituting GM-Lac were tested individually for antimicrobial activity in vitro. Then, GM-Lac was incorporated in fish feed at a dose of 5 × 106 cfu (g feed)−1 and fed to Asian seabass (Lates calcarifer) juveniles for eight weeks to assess the effects on growth performance, gastrointestinal (GI) histology and microflora, muscle amino acid composition, and disease resistance. According to the result of the zone of inhibition test, each GMNL strain presented noticeable antimicrobial activity against bacterial pathogens common in aquaculture. After the 8-week feeding trial, the fish in the GM-Lac group had higher final weight (p |
