Development of primary cell culture from the caudal fin region of snow trout Schizothorax plagiostomus (Heckel)

Autor:	Ashish K. Chowdhary, S N Bahuguna
Jazyk:	angličtina
Rok vydání:	2014
Předmět:	explant technique fetal bovine serum leibovitz-15media primary cell culture Schizothorax plagiostomus Environmental sciences GE1-350
Zdroj:	Environment Conservation Journal, Vol 15, Iss 1&2 (2014)
Druh dokumentu:	article
ISSN:	0972-3099 2278-5124
DOI:	10.36953/ECJ.2014.151219
Popis:	A primary cell culture has been established from the Snow trout Schizothorax plagiostomus. Primary cultures have developed from caudal fin tissues by explant culture technique. Cell outgrowth has been obtained from the caudal fin explant after 5-7 days of explant culture. The culture medium used for the experiment was Leibovitz-15 supplemented with 20% Fetal Bovine Serum. Radiation of cells from the explants started after 2 to 3 days. Both fibroblast like and epithelial like cells were noticed but fibroblast like cells dominated as the growth progressed.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/6fa7e1dc8802461a978ac2d37b76e62c Zobrazit plný text záznamu View record in DOAJ