Results of isolation and genotyping of measles viruses that circulated in 2012–2017 in the Odesa region
| Autor: | T.L. Hrydina, V.O. Honcharov, L.S. Kotlik, O.V. Skopenko, O.A. Hruzevsky, K.V. Radkevich |
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| Jazyk: | English<br />Ukrainian |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Aktualʹnaâ Infektologiâ, Vol 9, Iss 5-6, Pp 27-32 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2312-413X 2312-4148 |
| DOI: | 10.22141/2312-413X.9.5-6.2021.246693 |
| Popis: | Background. The circulation of different strains of the measles virus is closely related to the region and the incidence rate since circulating strains can change during epidemic outbreaks and in interepidemic periods. According to the WHO, the B3 strain is most common during outbreaks worldwide. Therefore, typing of circulating strains of measles virus, especially during an epidemic outbreak, is an important process, including for predicting the development of an epidemic. The study was aimed to identify and determine the genotype of measles virus types that circulate in Ukraine during 2012–2019. Materials and methods. Materials of the reporting documentation of the State Institution “Odessa Regional Laboratory Center of the Ministry of Health of Ukraine” in the Odessa region during 2012–2019 were used and analyzed. Materials from patients with suspected measles were used for molecular biological, genetic, analytical, and statistical approaches investigation. Following the standard WHO protocol for sequencing and phylogenetic analysis, circulating measles virus strains were isolated from the patient in a special culture of Vero/SLAM cells. Measles virus RNA was isolated from the resulting virus-containing material after cultivation and RT-PCR was performed. The resulting cDNA was sent for genotyping, which was carried out at the WHO reference laboratory for the diagnosis of measles and rubella in Luxembourg (WHO RRL). Results. Twenty strains of measles virus from 45 samples (urine and nasopharyngeal swabs) from patients diagnosed with measles were isolated during 2012–2014. Virus isolation was not carried out in 2015–2016 due to isolated cases of the disease. Twenty-four virus strains from 164 samples were isolated in 2017. Conclusions. The results obtained at the State Institution “Odessa Regional Laboratory Center” demonstrated that during the interepidemic period of 2012–2014, the D4 genotype circulated in the region. But since 2017, when there was an increas of cases associated with a new epidemic outbreak, B3, genetic line MVs/Kabul.AFG/20.2014/3 B3 mainly circulates in the region of southern Ukraine. As you can see, these data completely coincide with the data about circulating genotypes that were found at a certain time in the European Region, according to the data from the literature. |
| Databáze: | Directory of Open Access Journals |
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