CXCR4pos circulating progenitor cells coexpressing monocytic and endothelial markers correlating with fibrotic clinical features are present in the peripheral blood of patients affected by systemic sclerosis
Autor: | Diana Campioni, Andrea Lo Monaco, Francesco Lanza, Sabrina Moretti, Luisa Ferrari, Maria Fotinidi, Renato La Corte, Antonio Cuneo, Francesco Trotta |
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Jazyk: | angličtina |
Rok vydání: | 2008 |
Předmět: | |
Zdroj: | Haematologica, Vol 93, Iss 8 (2008) |
Druh dokumentu: | article |
ISSN: | 0390-6078 1592-8721 |
DOI: | 10.3324/haematol.12526 |
Popis: | There is still controversy regarding the role of circulating endothelial and progenitor cells (CECs/CEPs) in the pathogenesis of systemic sclerosis (SSc). Using a sequential Boolean gating strategy based on a 4-color flow cytometric protocol, an increased number of CD31pos/CD184pos(CXCR4)/CD34pos/CD45pos and CD31pos/CD117pos (c-kit-R) /CD34pos/ CD45pos hematopoietic circulating progenitor cells (HCPCs) was detected in SSc patients compared with healthy subjects. In SSc, no circulating mature and progenitor endothelial cells were observed, while an enhanced generation of erythroid progenitor cells was found to be correlated with the presence of CD117+ HCPCs. The presence of freshly detected CXCR4posHCPC was correlated either to the in vitro cultured spindle-shaped endothelial like cells (SELC) with an endo/myelomonocytic profile or to SDF-1 and VEGF serum level. These data are related to more fibrotic clinical features of the disease, thus supporting a possible role of these cells in fibrosis. |
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