Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of Toxoplasma gondii antibodies in giant panda (Ailuropoda melanoleuca)

Autor: Chanjuan Yue, Wanjing Yang, Yunli Li, Dongsheng Zhang, Jingchao Lan, Xiaoyan Su, Lin Li, Yiyan Liu, Weichao Zheng, Kongju Wu, Xueyang Fan, Xia Yan, Rong Hou, Songrui Liu
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal for Parasitology: Parasites and Wildlife, Vol 18, Iss , Pp 287-291 (2022)
Druh dokumentu: article
ISSN: 2213-2244
DOI: 10.1016/j.ijppaw.2022.07.001
Popis: Toxoplasma gondii is a worldwide-distributed zoonotic protozoan parasite which causes toxoplasmosis and has a significant effect on public health. In the giant panda (Ailuropoda melanoleuca), toxoplasmosis can cause asymptomatic infections, reproductive disorder and even death, which poses a serious threat to the conservation of this rare protected species. Therefore, serological investigation of T. gondii is essential to understanding its risk to giant pandas, however, there are no specific testing kits for giant pandas. Previous research has used MAT as the reference method for screening T. gondii, to investigate this further, this study focused on the agreement comparing of MAT with ELISA and IHA tests for detecting T. gondii antibodies in 100 blood samples from 55 captive giant pandas in Chengdu, China. The results showed 87.0%, 87.0%, 84.0%, samples were sero-positive for T. gondii using ELISA (kits a, b, c), respectively, while MAT and IHA tests were 84.0% and 9.0% sero-positive, respectively. There was no significant difference between MAT and the three ELISA kits and these two methods had substantial agreement (0.61
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