Improvement of eggshell quality by dietary N-carbamylglutamate supplementation in laying chickens

Autor: Yanfen Ma, Jinwei Yao, Shuo Zhou, Yuling Mi, Jian Li, Caiqiao Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Poultry Science, Vol 99, Iss 8, Pp 4085-4095 (2020)
Druh dokumentu: article
ISSN: 0032-5791
DOI: 10.1016/j.psj.2020.04.004
Popis: Egg quality defects seriously reduce the quality grade and increase egg breakage in egg marketing activities. In this study, the effect of N-carbamylglutamate (NCG) on eggshell quality was investigated by evaluating calcium absorption and calcification in laying hens. A total of 30 newly hatched female Hy-Line chicks were randomly assigned to the control group (basal diet) and treatment group (basal diet supplemented with 1% NCG). At 25 wk, eggs from each group were obtained to assess egg quality parameters. Blood samples were collected for analysis of mineral, hormone, and amino acids levels at 16 h after laying egg. Uterine tissues were removed and fixed in 4% neutral paraformaldehyde solution or kept in liquid nitrogen for mineral determination, quantitative PCR, and Western blot. Results showed that the egg quality (eggshell thickness, strength and percentage, egg specific gravity, and eggshell effective thickness) was significantly increased while effective thickness of mastoid layer, width of mastoid gap, and mammillary knobs were significantly decreased by dietary NCG supplementation (P
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