MACS-W: A modified optical clearing agent for imaging 3D cell cultures

Autor: Xiang Zhong, Chao Gao, Hui Li, Yuening He, Peng Fei, Zaozao Chen, Zhongze Gu, Dan Zhu, Tingting Yu
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Innovative Optical Health Sciences, Vol 17, Iss 02 (2024)
Druh dokumentu: article
ISSN: 17935458
1793-7205
1793-5458
DOI: 10.1142/S1793545823500189
Popis: Three-dimensional (3D) cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models. The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness. While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D, they often have limitations, such as being too harsh for fragile 3D cell cultures, requiring complex handling protocols, or inducing tissue deformation with shrinkage or expansion. To address this issue, we proposed a modified optical clearing method for 3D cell cultures, called MACS-W, which is simple, highly efficient, and morphology-preserving. In our evaluation of MACS-W, we found that it exhibits excellent clearing capability in just 10[Formula: see text]min, with minimal deformation, and helps drug evaluation on tumor spheroids. In summary, MACS-W is a fast, minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.
Databáze: Directory of Open Access Journals
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