| Autor: |
Chen Cao, Hui-min Liu, Wei Li, Yang Wu, Yan Leng, Rui Xue, Rong Chen, Ling-hua Tang, Qian Sun, Zhongyuan Xia, Qi-zhu Tang, Di-fei Shen, Qing-tao Meng |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Acta Cirúrgica Brasileira, Vol 35, Iss 1 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
0102-8650 |
| DOI: |
10.1590/s0102-865020200010000007 |
| Popis: |
Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton. Methods Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion). Myocardial infarct area was determined by TTC staining. Cardiac function was monitored by a microcatheter. ANP, 15-F2t-isoprostane, nitrotyrosine and MDA were measured by assay kits. Levels of p-Akt, total-Akt and GAPDH were determined by Western Blot. Results Diabetic rats subjected to myocardial IR exhibited severe myocardial infarction and oxidative stress injury, lower APN in the plasma and cardiac p-Akt expression ( P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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