An evaluation of antimicrobial potential of irreversible hydrocolloid impression material incorporated with chitosan

Autor: Yenumula J. B Manikyamba, A V. Rama Raju, M C. Suresh Sajjan, P Arun Bhupathi, D Bheemalingeswara Rao, Jampana V. V. S. N Raju
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: The Journal of Indian Prosthodontic Society, Vol 20, Iss 3, Pp 297-303 (2020)
Druh dokumentu: article
ISSN: 0972-4052
1998-4057
DOI: 10.4103/jips.jips_50_20
Popis: Aim: To evaluate the antimicrobial potential of irreversible hydrocolloid impression material manipulated using chitosan impregnated solution at various time intervals. Setting and Design: Evaluative invivo study design. Materials and Methods: Maxillary impressions made for 20 dentulous volunteers using irreversible hydrocolloid impression material manipulated using distilled water as control and using 1% chitosan impregnated solution as test group using stock metal trays with one-week interval. Bacterial samples were collected using dry sterile cotton swab in the mid-palatal region at the time intervals of 0, 10, 20 minutes. Bacterial swabs were inoculated on nutrient agar media and incubated at 37° C for 24 hours. Bacterial colonies were counted with the aid of colony counter. Statistical Analysis Used: The resultant data was subjected to statistical analysis using repeated measures ANOVA and independent t test. Results: Adding water soluble chitosan to irreversible hydrocolloid impression material resulted in superior antimicrobial activity. With the passage of time there was a significant decrease in the microbial colony count upto 10min (p=0.016). However, the rate of decrease of microbial colony count was statistically insignificant between the samples collected at 10 and 20 min. Conclusion: Incorporation of water soluble chitosan to irreversible hydrocolloid impression material showed significant antimicrobial activity in 10 minutes.
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