Evaluation of the BD Phoenix Carbapenemase-Producing Organism Panels for the Detection of Carbapenemase Producers in Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa

Autor: Yoselin Paola Correa-León, José Miguel Pérez-Hernández, Bernardo Alfonso Martinez-Guerra, Eduardo Rodríguez-Noriega, Juan Pablo Mena-Ramírez, Eduardo López-Gutiérrez, Luis Esaú López-Jácome, Víctor Antonio Monroy-Colin, Christian Daniel Mireles-Davalos, Cecilia Padilla-Ibarra, María Angelina Quevedo-Ramos, José Manuel Feliciano-Guzmán, Talía Pérez-Vicelis, María del Consuelo Velázquez-Acosta, Melissa Hernández-Durán, Elvira Garza-González
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Diagnostics, Vol 13, Iss 22, p 3417 (2023)
Druh dokumentu: article
ISSN: 2075-4418
DOI: 10.3390/diagnostics13223417
Popis: The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico. Carbapenem non-susceptible isolates collected in Mexico were included. The clinical isolates (n = 484) comprised Klebsiella pneumoniae (n = 154), Escherichia coli (n = 150), and P. aeruginosa (n = 180). BD Phoenix CPO NMIC-504 and NMIC-501 panels were used for the identification of species, antimicrobial susceptibility tests, and detection of CPOs. For the detection of carbapenemase-encoding genes, E. coli and K. pneumoniae were evaluated using PCR assays for blaNDM-1, blaKPC, blaVIM, blaIMP, and blaOXA-48-like. For P. aeruginosa, blaVIM, blaIMP, and blaGES were detected using PCR. Regarding E. coli, the CPO panels had a sensitivity of 70% and specificity of 83.33% for the detection of a class B carbapenemase (blaNDM in the molecular test). Regarding K. pneumoniae, the panels had a sensitivity of 75% and specificity of 100% for the detection of a class A carbapenemase (blaKPC in the molecular test). The Phoenix NMIC-501 panels are reliable for detecting class B carbapenemases in E. coli. The carbapenemase classification in K. pneumoniae for class A carbapenemases has a high specificity and PPV; thus, a positive result is of high value.
Databáze: Directory of Open Access Journals
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