Suitability of melanoma FFPE samples for NGS libraries: time and quality thresholds for downstream molecular tests
| Autor: | David Millán-Esteban, Diana Reyes-García, Zaida García-Casado, José Bañuls, José Antonio López-Guerrero, Celia Requena, Aranzazu Rodríguez-Hernández, Víctor Traves, Eduardo Nagore |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 65, Iss 2, Pp 79-85 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2018-0016 |
| Popis: | The use of NGS in clinical practice for precision diagnosis requires a quality starting material. Despite the broadly established use of formalin-fixed paraffin-embedded (FFPE) samples in molecular testing, these usually have low-quality DNA. We established a method to determine the suitability of melanoma FFPE samples for an amplicon-based NGS custom panel analysis. DNA was extracted from unstained melanoma samples and wide local excision samples. Amplicon-based libraries were constructed and tested using time and quality parameters as variables. Time elapsed from sample retrieval >7 years, a quality control value > 5.63 and a DNA integrity value |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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