Tissue microarray technology and collagen evaluation to analyze surgical trauma performed with usual blade or ultrasonic harmonic scalpels in rats

Autor: Octavio Antonio Azevedo da Costa-Filho, Mario Augusto Cray da Costa, Ana Maria Waaga-Gasser, Luiz Fernando Kubrusly, Luciane Bugmann Moreira de Oliveira, Luiz Martins Collaço, Maria Angélica Baron Magalhães, Martin Gasser, Osvaldo Malafaia, Jurandir Marcondes Ribas-Filho
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Acta Cirúrgica Brasileira, Vol 33, Iss 9, Pp 762-774 (2018)
Druh dokumentu: article
ISSN: 0102-8650
DOI: 10.1590/s0102-865020180090000004
Popis: Abstract Purpose: To compare wound healing performed with cold blade (CSB) and ultrasonic harmonic scalpel (UHS) in the abdominal aponeurosis of rats. Methods: Eighty Wistar rats divided into two groups and underwent midline incision in the linea alba with cold blade and harmonic ultrasonic scalpel. Analysis were performed in subgroups of 10 animals after 3, 7, 14 and 21 days. Macroscopically was observed the presence of hematoma, infection, wound dehiscence, fistula and adherences. Microscopically were used collagen and immunohistochemical staining methods. Results: Macroscopic, complications showed no statistical difference. Immunohistochemical analysis for MMP-9 was more intense in UHS group (p
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