Chlorophyll fluorescence and biomass partitioning within light and nitrogen deficiency: An example of the use of the R programming language for teaching

Autor: Junior Pastor Pérez-Molina, Raquel Castro Lara, Ian Portuguez Brenes, Valerie Araya Trejos, Andrey Quesada Traña
Jazyk: English<br />Spanish; Castilian
Rok vydání: 2020
Předmět:
Zdroj: Cuadernos de investigación UNED, Vol 12, Iss 1 (2020)
Druh dokumentu: article
ISSN: 1659-4266
1659-441X
DOI: 10.22458/urj.v12i1.2629
Popis: Introduction: Chlorophyll Fluorescence (Fv/Fm) detects damage in photosystem II (PSII) in plants exposed to stress (biotic and/or abiotic). Objective: In this context, Fv/Fm for the diagnosis of the effect of N deficiency and light in tomato plants (Solanum lycopersicum) was evaluated. Methods: We used the software R to analyze data from an experiment consisted of two light treatments (high and low) and two Hoagland nutritive solutions (N deficiency: (-)N; and complete solution: C). Twenty tomato plants within the two light treatments, with two nutrient levels ((-)N and C) were used. For four weeks, weekly measurements for height (H), leaf number (NL), and Fv/Fm were made. On the fourth week, the dry mass partitioning (root: RMR; stem: SMR; and leaves: LMR) and chlorophyll concentration (a, b, a/b, and total) was measured. Results: We found statistically significant differences between treatments for H, NL and Fv/Fm (KW> 14,5, g.l.= 3,76, P0,05). In both light conditions it was higher LMR in C treatment, but with lower RMR compared to (-)N. Statistically significant correlations (% Spearman, P
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