| Autor: |
Meghana Natesh, Ryan Taylor, Stefan Prost, Elizabeth Hadly, Dmitri Petrov, Uma Ramakrishnan |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Canadian Journal of Biotechnology, Vol 1, Iss Special Issue, Pp 19-19 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2560-8304 |
| DOI: |
10.24870/cjb.2017-a7 |
| Popis: |
Tigers have experienced dramatic range contraction in the recent past and currently occupy only 7% of their historical range [1]. Genetic tools can be used effectively to monitor wild species of conservation concern such as tigers. Such approaches allow us to identify individuals, reconstruct relatedness between them, and monitor connectivity between populations. Microsatellite markers are currently used across many laboratories to study tiger population and conservation genetics. However, non-invasive samples such as feces continue to present challenges with high error rates and low amplification success for such microsatellite loci [2]. In this study, we developed a panel of Single Nucleotide Polymorphism (SNP) markers and experimental pipeline for use with fecal samples from wild tigers. Multiplex PCR followed by Illumina sequencing of pooled, barcoded samples allowed fast implementation of these protocols. A total of 339 SNPs were targeted and amplified in short fragments of 40 base pairs. All samples were run in triplicate to investigate error among replicates. In the first run the protocol was tested using captive tiger fecal samples of different ancestry and a varying target DNA concentration. Following this, non-invasive samples (fecal, saliva and shed hair) collected from multiple field sites across India were tested. Results revealed that samples with very low initial concentration of target DNA ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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